Hello folks, Quick question. I have RNAseq count data sets which I would like to analyze together. Experimentall they are similair they only differ by their depths of coverage . One set has about 30 million reads and the other has about 70 million. When you look at these files in an MDS plot you can see they are clearly different. Can anyone recommend a good aggressive normalization technique? Something similar to combat? I have tried the built in ones in DEseq and EDGER but would like to use one that's slightly more aggressive. Any suggestions are appreciated .thanks
-Rich
-Rich
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