Could anybody help me to evaluate my transcriptome?
I have now MIRA and Cap3 assembled tick (Amblyomma americanum) transcriptome, which was sequenced by 454 pyrosequencing.
I would like to evaluate my transcriptome by comparing gold-standard such as genome. Unfortunately, there is no genome sequence for Amblyomma americanum; however, there is transcriptomes from NCBI. Do you think I can evaluate my transcriptome by comparing with previous transcriptomes? Or Could you give me any idea to evaluate my transcriptome and how to do it?
Thank you very much in advance.
I have now MIRA and Cap3 assembled tick (Amblyomma americanum) transcriptome, which was sequenced by 454 pyrosequencing.
I would like to evaluate my transcriptome by comparing gold-standard such as genome. Unfortunately, there is no genome sequence for Amblyomma americanum; however, there is transcriptomes from NCBI. Do you think I can evaluate my transcriptome by comparing with previous transcriptomes? Or Could you give me any idea to evaluate my transcriptome and how to do it?
Thank you very much in advance.
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