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  • Greg
    Member
    • Oct 2009
    • 31

    tophat errors

    Does anyone know where there is a list of what all the tophat errors mean?

    For example, I have been getting:
    ...
    [Tue Jan 12 09:04:36 2010] Searching for junctions via segment mapping
    [FAILED]
    Error: segment-based junction search failed with err = -6
    when trying to align some 32bp illumina reads. What does error -6 mean? (or 1-5 for that matter) If I run truncated versions of the same data set (up to under a million reads or so) it will run without error but the junction file will be empty.

    much appreciated.

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
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    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
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    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

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