Hi everybody im new y this, im from mexico and i have a data from total RNAm in one condition of the bacteria R. etli the first what i see is tha some intergenic region got a read, that the orfs have diferents numbers of reads.
the cuestion is how can i normalize the data? of the RNA-seq
if i have to use RPKM?
is exist any tool to normalize the results from teh illumina?
remember that i just have the results of one condition and i think that i have to normalize this data to next compare whit other condition.
thank you everybody for your time and im sorry for the spell
the cuestion is how can i normalize the data? of the RNA-seq
if i have to use RPKM?
is exist any tool to normalize the results from teh illumina?
remember that i just have the results of one condition and i think that i have to normalize this data to next compare whit other condition.
thank you everybody for your time and im sorry for the spell
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