Seqanswers Leaderboard Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • yl01
    started a topic Invalid VCF files from Lifescope - allele "-"

    Invalid VCF files from Lifescope - allele "-"

    I found some problematic VCF files created by Lifescope software. The sequencing was done on Solid 5500. These VCFs cannot be merged with other VCF files. The variants in these files are all indels. When I run vcf-validator it seems the "-" in the allele is the problem. Some vcf-validator output:

    [lifescope@scyld small.indel]$ perl -I/home/lifescope/bin/vcftools_0.1.11/perl /home/lifescope/bin/vcftools_0.1.11/bin/vcf-validator 37_1_3/2013_08_13_exome_high.gff3.vcf
    Warning: The file is not sorted, for example chr12:53690189 comes after chr12:53690191
    chr12:53690189 .. Could not parse the allele(s) [-]
    [lifescope@scyld small.indel]$ perl -I/home/lifescope/bin/vcftools_0.1.11/perl /home/lifescope/bin/vcftools_0.1.11/bin/vcf-validator 37_1_4/2013_08_13_exome_high.gff3.vcf
    Warning: The file is not sorted, for example chr11:108100105 comes after chr11:108100112
    chr11:108100105 .. Expected combination of A,C,G,T,N for REF, got [-], the offending chars were [-]

    Does any other Lifescope user have the same problem? Is there an easy way to fix this? Thank you in advance!

Latest Articles

Collapse

  • seqadmin
    New Genomics Tools and Methods Shared at AGBT 2025
    by seqadmin


    This year’s Advances in Genome Biology and Technology (AGBT) General Meeting commemorated the 25th anniversary of the event at its original venue on Marco Island, Florida. While this year’s event didn’t include high-profile musical performances, the industry announcements and cutting-edge research still drew the attention of leading scientists.

    The Headliner
    The biggest announcement was Roche stepping back into the sequencing platform market. In the years since...
    03-03-2025, 01:39 PM
  • seqadmin
    Investigating the Gut Microbiome Through Diet and Spatial Biology
    by seqadmin




    The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...
    02-24-2025, 06:31 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 03-20-2025, 05:03 AM
0 responses
17 views
0 reactions
Last Post seqadmin  
Started by seqadmin, 03-19-2025, 07:27 AM
0 responses
18 views
0 reactions
Last Post seqadmin  
Started by seqadmin, 03-18-2025, 12:50 PM
0 responses
19 views
0 reactions
Last Post seqadmin  
Started by seqadmin, 03-03-2025, 01:15 PM
0 responses
186 views
0 reactions
Last Post seqadmin  
Working...