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Well, the minimum number would be 2, but you'll want to merge all of the samples involved in any comparison that you intend to make. -
Transcriptome Assembly Questions for Cuffmerge
Hi. I am trying to run a rather large experiment for my first RNAseq project. Unfortunately, I was convinced to use our SOLiD machines due to cost and proximity (but that's a different issue
). Let's say I get past this and have some mapped reads to input into Cufflinks and then Cuffmerge to make an assembly.
How many Assembled Transcripts from how many replicate samples are required as input to Cuffmerge to make the final assembly? Do I need to dump ALL of the Assembled Transcripts for each experiment?
The experiment is using three genotypes (Control, Del, Dup) and three biological replicates per genotype (R1, R2, R3). In addition, we are using both DPSCs and DPSC-neurons for all of these conditions. I said it was big!
Any help appreciated.
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The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...08-27-2024, 04:44 AM -
Sequencing mRNA provides a snapshot of cellular activity, allowing researchers to study the dynamics of cellular processes, compare gene expression across different tissue types, and gain insights into the mechanisms of complex diseases. “mRNA’s central role in the dogma of molecular biology makes it a logical and relevant focus for transcriptomic studies,” stated Sebastian Aguilar Pierlé, Ph.D., Application Development Lead at Inorevia. “One of the major hurdles for...08-07-2024, 12:11 PM
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