-
Well, the minimum number would be 2, but you'll want to merge all of the samples involved in any comparison that you intend to make. -
Transcriptome Assembly Questions for Cuffmerge
Hi. I am trying to run a rather large experiment for my first RNAseq project. Unfortunately, I was convinced to use our SOLiD machines due to cost and proximity (but that's a different issue
). Let's say I get past this and have some mapped reads to input into Cufflinks and then Cuffmerge to make an assembly.
How many Assembled Transcripts from how many replicate samples are required as input to Cuffmerge to make the final assembly? Do I need to dump ALL of the Assembled Transcripts for each experiment?
The experiment is using three genotypes (Control, Del, Dup) and three biological replicates per genotype (R1, R2, R3). In addition, we are using both DPSCs and DPSC-neurons for all of these conditions. I said it was big!
Any help appreciated.
-
Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...09-23-2024, 06:35 AM -
During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.
Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...09-09-2024, 10:59 AM
Leave a comment: