Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Well, the minimum number would be 2, but you'll want to merge all of the samples involved in any comparison that you intend to make.
-
Transcriptome Assembly Questions for Cuffmerge
Hi. I am trying to run a rather large experiment for my first RNAseq project. Unfortunately, I was convinced to use our SOLiD machines due to cost and proximity (but that's a different issue ). Let's say I get past this and have some mapped reads to input into Cufflinks and then Cuffmerge to make an assembly.
How many Assembled Transcripts from how many replicate samples are required as input to Cuffmerge to make the final assembly? Do I need to dump ALL of the Assembled Transcripts for each experiment?
The experiment is using three genotypes (Control, Del, Dup) and three biological replicates per genotype (R1, R2, R3). In addition, we are using both DPSCs and DPSC-neurons for all of these conditions. I said it was big!
Any help appreciated.Tags: None
Latest Articles
Collapse
-
by seqadmin
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
Channel: Articles
03-22-2024, 06:39 AM -
-
by seqadmin
The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.
Avian Conservation
Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...-
Channel: Articles
03-08-2024, 10:41 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 03-27-2024, 06:37 PM
|
0 responses
13 views
0 likes
|
Last Post
by seqadmin
03-27-2024, 06:37 PM
|
||
Started by seqadmin, 03-27-2024, 06:07 PM
|
0 responses
12 views
0 likes
|
Last Post
by seqadmin
03-27-2024, 06:07 PM
|
||
Started by seqadmin, 03-22-2024, 10:03 AM
|
0 responses
53 views
0 likes
|
Last Post
by seqadmin
03-22-2024, 10:03 AM
|
||
Started by seqadmin, 03-21-2024, 07:32 AM
|
0 responses
69 views
0 likes
|
Last Post
by seqadmin
03-21-2024, 07:32 AM
|
Leave a comment: