Hi,
I will receive soon mate pairs reads from illumina MiSeq. I will have the circularization adapter and need to trimm it (with cutadapt) from reads before assemble them de novo. Do I need to reverse complement them before the trimming step?
After, I will need to remove orphan reads, what do you think about the script filteresCommonPEreads.sh in this page:http://seqanswers.com/forums/showthr...oto=nextoldest?
Thanks!
I will receive soon mate pairs reads from illumina MiSeq. I will have the circularization adapter and need to trimm it (with cutadapt) from reads before assemble them de novo. Do I need to reverse complement them before the trimming step?
After, I will need to remove orphan reads, what do you think about the script filteresCommonPEreads.sh in this page:http://seqanswers.com/forums/showthr...oto=nextoldest?
Thanks!
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