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  • g781
    Member
    • May 2010
    • 25

    #1

    the question from pairToBed output

    Hi everyone,

    I was testing the pairToBed with version 2.17.0, but I was confused with the output.

    -- CASE 1 --
    Here is the input A in bedpe:
    Code:
    chrBB	100	230	chrBB	330	400	paired-read-1	60	+	-
    Here is the input B in bed6:
    Code:
    chrBB	200	250	gene1	1	+
    chrBB	300	350	gene1	1	+
    Here is the command for running pairToBed:
    Code:
    pairToBed -a A.bedpe -b B.bed6 -bedpe -type both
    Here is the outputs:
    Code:
    chrBB   100     230     chrBB   330     400     paired-read-1   60      +       -       chrBB   200     250     gene1   1       +
    chrBB   100     230     chrBB   330     400     paired-read-1   60      +       -       chrBB   300     350     gene1   1       +

    -- CASE 2 --
    Here is the input A in bedpe:
    Code:
    chrBB	100	230	chrBB	330	400	paired-read-1	60	+	-
    Here is the input B in bed6:
    Code:
    chrBB	200	350	gene1	1	+
    Here is the command for running pairToBed:
    Code:
    pairToBed -a A.bedpe -b B.bed6 -bedpe -type both
    Here is the outputs:
    Code:
    chrBB   100     230     chrBB   330     400     paired-read-1   60      +       -       chrBB   200     250     gene1   1       +
    chrBB   200     350     chrBB   330     400     paired-read-1   60      +       -       chrBB   200     350     gene1   1       +

    I would expect just one record. But both case seem to me, pairToBed would show the two records for each read in PE-read. Is that correct?

    Thanks.

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