Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Time
  • Show
Clear All
new posts
Previous template Next
  • Fernas
    Member
    • Apr 2013
    • 74
    #1

    detect Isoforms using Cufflinks and Cuffdiff

    Dear all,

    I am using cufflinks and cuffdiff to detect different isoforms of gene from some CAGE data. I got very few number of isoforms. I expected to find much more. What is the parameters that you think I should change and play with in order to detect all possible isoforms.
    Note: I have two groups of samples, each group has 5 biological replicates.
    Tags: cuffdiff isoforms
    Previous template Next

    Latest Articles

    Collapse
    • SEQadmin2
      Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
      by SEQadmin2



      CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

      Despite this, “CRISPR helped turn genome editing from a specialized technique into      ...
      Yesterday, 11:01 AM
    • SEQadmin2
      Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
      by SEQadmin2


      Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

      The systematic characterization of the human proteome has      ...
      07-20-2026, 11:48 AM
    • SEQadmin2
      Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
      by SEQadmin2



      Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
      ...
      07-09-2026, 11:10 AM
    View All

    ad_right_rmr

    Collapse

    News

    Collapse
    Topics Statistics Last Post
    New Genomic Method Uncovers Ancient Hominin DNA by SEQadmin2
    Started by SEQadmin2, Yesterday, 02:55 AM
    0 responses
    9 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    Yesterday, 02:55 AM  
    Study Captures the First Moments of DNA Replication by SEQadmin2
    Started by SEQadmin2, 07-24-2026, 12:17 PM
    0 responses
    12 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    07-24-2026, 12:17 PM  
    Chemotherapy Leaves Detectable DNA Signatures in Childhood Tumors by SEQadmin2
    Started by SEQadmin2, 07-23-2026, 11:41 AM
    0 responses
    12 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    07-23-2026, 11:41 AM  
    Single-Cell Atlases Skew Toward European Ancestry, Analysis Finds by SEQadmin2
    Started by SEQadmin2, 07-20-2026, 11:10 AM
    0 responses
    24 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    07-20-2026, 11:10 AM  
    View All
    Working...