Hello !
I need to add read groups to my BAM files before merging and variant calling with GATK.
Do you have any advice ?
I will use AddOrReplaceReadGroups.jar (picard) and plan to fill in the following tags : ID, library (LB), sequencing platform=Illumina (PL), and sample (SM).
Am I missing another important tag ?
Also, if I understand well, the ID should be unique to a sample, lane and sequencing experiment ?
Thanks,
Muriel
I need to add read groups to my BAM files before merging and variant calling with GATK.
Do you have any advice ?
I will use AddOrReplaceReadGroups.jar (picard) and plan to fill in the following tags : ID, library (LB), sequencing platform=Illumina (PL), and sample (SM).
Am I missing another important tag ?
Also, if I understand well, the ID should be unique to a sample, lane and sequencing experiment ?
Thanks,
Muriel
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