I'm new to bioinformatics so I apologize in advance. I have a pipeline for genome assembly, the output I get is a de novo assembly file SPAdes, contigs.fasta, as well as a variant file vcf.fasta, I need to use the information about the variants from vcf.fasta and make these changes to contig.fasta, correct do I think? if yes, how can I do it better? I need to somehow combine information from vcf.fast and contigs.fast to get a consensus sequence.
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While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...-
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