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I'm new to bioinformatics so I apologize in advance. I have a pipeline for genome assembly, the output I get is a de novo assembly file SPAdes, contigs.fasta, as well as a variant file vcf.fasta, I need to use the information about the variants from vcf.fasta and make these changes to contig.fasta, correct do I think? if yes, how can I do it better? I need to somehow combine information from vcf.fast and contigs.fast to get a consensus sequence.
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Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...10-18-2024, 07:11 AM -
Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.
Nobel Prize for MicroRNA Discovery
This week,...10-07-2024, 08:07 AM