Hi,
I am really new in NGS but I have looked for RNAseq data, aligned with tophat. I used then picard to look at different statistics on this dataset and specifficaly what is called "insert size". It should be define as the distance between two potential mate pair.
First : How mate pair are linked ?
Second : What is usually do with this data ?
But my main question regarding this histogram :
When an insert size is big, it could mean the gene in dna contain big intronic region. Where is it discuss ? What do you think about that ?
I would enjoy any comment on this !
I am really new in NGS but I have looked for RNAseq data, aligned with tophat. I used then picard to look at different statistics on this dataset and specifficaly what is called "insert size". It should be define as the distance between two potential mate pair.
First : How mate pair are linked ?
Second : What is usually do with this data ?
But my main question regarding this histogram :
When an insert size is big, it could mean the gene in dna contain big intronic region. Where is it discuss ? What do you think about that ?
I would enjoy any comment on this !
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