Hi,
Question 1
Does Ray support Illumina 1.6+ fastq format (with trailing B's)?
Answer
No, but it should work if the trailing B's are at the end, but not at the beginning.
Question 2
Does Ray have the capability for trimming low-quality bases or should I pre-process my reads beforehand?
Answer
Ray does not trim sequences, but random errors are not a problem.
Question 3
Should I convert my libraries to Phred/Sanger scores?
Answer
No: Ray does not use quality scores.
Question 4
Can I run Bambus with Rays's output?
Answer
Ray outputs a fasta file -- I have never utilized Bambus, so the question is what Bambus needs.
-Sebhtml
Question 1
Does Ray support Illumina 1.6+ fastq format (with trailing B's)?
Answer
No, but it should work if the trailing B's are at the end, but not at the beginning.
Question 2
Does Ray have the capability for trimming low-quality bases or should I pre-process my reads beforehand?
Answer
Ray does not trim sequences, but random errors are not a problem.
Question 3
Should I convert my libraries to Phred/Sanger scores?
Answer
No: Ray does not use quality scores.
Question 4
Can I run Bambus with Rays's output?
Answer
Ray outputs a fasta file -- I have never utilized Bambus, so the question is what Bambus needs.
-Sebhtml
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