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  • De novo assembly of a small region after using PINDEL?

    Hello again,
    I have just recently got the program PINDEL to work on my reads, and it identified a large deletion (9kb) in my region of interest (gene family, roughly 40kb). What I would really love, is to take all the reads from PINDEL, and my original alignment from that particular area, and re-align them without the reference, to see if it can make sense of the region. Has anyone had any luck doing such a thing? If so, can you point me in the right direction?
    Thanks so much
    A

    /I've also already designed primers to test if this deletion is legit, so that's obviously a first step.

  • #2
    Try Tigra-SV


    An international, peer-reviewed genome sciences journal featuring outstanding original research that offers novel insights into the biology of all organisms

    Comment


    • #3
      Tigra looks interesting, but from what I can tell that version is no longer being developed, and the original author now has version 0.3.9 here:



      Also, neither of these will compile with samtools 0.1.19, but 0.1.18 works.
      For the benefit of Google searchers, the error message with 0.1.19 is
      /usr/lib/samtools/libbam.a(bgzf.o): In function `bgzf_mt':
      .../samtools-0.1.19/bgzf.c:445: undefined reference to `pthread_create'
      /usr/lib/samtools/libbam.a(bgzf.o): In function `mt_destroy':
      .../samtools-0.1.19/bgzf.c:458: undefined reference to `pthread_join'

      Comment

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