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  • anecsulea
    replied
    I don't know what the problem was, but I think I did find a solution: see
    Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc


    Let me know if you need more help on this.

    Originally posted by Ms@New View Post
    Hi, I get the same error, and I'm using hg18 index from bowtie website.

    Did you figure out what was the problem?

    Leave a comment:


  • Ms@New
    replied
    Hi, I get the same error, and I'm using hg18 index from bowtie website.

    Did you figure out what was the problem?

    Leave a comment:


  • anecsulea
    replied
    No, this can't be it... For me, this error occurs while running TopHat, and in the TopHat run the fasta file and the bowtie index files are in the same directory.

    Leave a comment:


  • Nicolas902
    replied
    If I remember well, this was a question of directory. I moved the chromosomes sequences I used to the "bowtie" directory.

    I hope this was the solution, tell me if it makes it for you.

    Leave a comment:


  • anecsulea
    replied
    Hi,

    I've been having a similar error. I'm building a bowtie index for a genome sequence, bowtie-build finishes without error, but when trying to align reads with bowtie I get this error:

    "Error reading ebwt array: returned 41750080, length was 168445184
    Your index files may be corrupt; please try re-building or re-downloading. "

    Have you found a solution to the problem ? All suggestions would be appreciated.

    Best,

    Anamaria

    Leave a comment:


  • Nicolas902
    started a topic bowtie error "Error reading _offs[] array: "

    bowtie error "Error reading _offs[] array: "

    Dear all,

    I am struggling with a bowtie error which occurs in the ebwt.h, here is the code:

    Code:
    else {
    						// Do it all in one read
    						MM_READ_RET r = MM_READ(_in2, (void*)this->_offs, offsLen << 2);
    						if(r != (MM_READ_RET)(offsLen << 2)) {
    							cerr << "Error reading _offs[] array: " << r << ", " << (offsLen << 2) << endl;
    							throw 1;
    						}
    					}
    I have built an index using a regular fasta file of hg18 downloaded form ucsc and a splice.fa file generated using ERANGE. bowtie-build is running without any error, as well as "getsplicefa.py" which generates the splice file.

    Any idea what this error means?

    Thank you.

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