Hi
I am very beginner in Bioinformatics especially sequencing method.
Now I just read some paper (Title : De novo assembly of human genomes with massively parallel short read sequencing)
So I can't understand several terms in the paper. So I need your help.
In the paper contents,
"Resolving the repeat clusters between unique clusters and assembling them
into an intact sequence requires the paired-end relationship of a pair of short
reads generated from both ends of a DNA clone"
In this paragraph, I can't figure out that the term repeat clusters and unique clusters is indicating about. I have learned that cluster is just a action that gathers something similar. But in this paper, what is the repeat clusters and unique cluster meaning?
And also, I am not sure of meaning of paired-end method.
Since I am very beginner in this areas, so I am looking forward to hearing some easily explain about my questions.
Thank you
I am very beginner in Bioinformatics especially sequencing method.
Now I just read some paper (Title : De novo assembly of human genomes with massively parallel short read sequencing)
So I can't understand several terms in the paper. So I need your help.
In the paper contents,
"Resolving the repeat clusters between unique clusters and assembling them
into an intact sequence requires the paired-end relationship of a pair of short
reads generated from both ends of a DNA clone"
In this paragraph, I can't figure out that the term repeat clusters and unique clusters is indicating about. I have learned that cluster is just a action that gathers something similar. But in this paper, what is the repeat clusters and unique cluster meaning?
And also, I am not sure of meaning of paired-end method.
Since I am very beginner in this areas, so I am looking forward to hearing some easily explain about my questions.
Thank you