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  • sunnyvu
    Member
    • Mar 2010
    • 17

    #1

    TopHat "accepted_hits.sam" header line

    I am runing the TopHat. I found that the header line of the file "accepted_hits.sam"is as following:
    @HD VN:1.0 SO:sorted
    @PG TopHat VN:1.0.130

    The file "accepted_hits.sam" does not working for Samtools.

    I use the commond "./samtools view -S -t -X -o exampl.flag accepted_hits.sam", and got two errors:

    The tag [ID] required for [PG] not present.
    Segmentation fault..

    According to suggestions from nilshomer, I changed the line "@PG ID:TopHat VN:1.0.13). The first error is gone.

    So my problems:
    1) Why my file "accepted_hits.sam" without "ID:"?
    2) how to fix the second error?

    Thanks
  • nilshomer
    Nils Homer
    • Nov 2008
    • 1283

    #2
    Originally posted by sunnyvu View Post
    I am runing the TopHat. I found that the header line of the file "accepted_hits.sam"is as following:
    @HD VN:1.0 SO:sorted
    @PG TopHat VN:1.0.130

    The file "accepted_hits.sam" does not working for Samtools.

    I use the commond "./samtools view -S -t -X -o exampl.flag accepted_hits.sam", and got two errors:

    The tag [ID] required for [PG] not present.
    Segmentation fault..

    According to suggestions from nilshomer, I changed the line "@PG ID:TopHat VN:1.0.13). The first error is gone.

    So my problems:
    1) Why my file "accepted_hits.sam" without "ID:"?
    2) how to fix the second error?

    Thanks
    It seems that it is still not liking the "@PG" line. Can you make sure that they are TAB separated?

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