Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Add a new line after the last file recording

    Dear all,

    I used ShortRead package to filter the data and then saved as fastq file. But when I run the qa function again there is error in .local(dirPath, pattern, ...): > > ShortRead internal: too many 'snap' entries.

    In the bioc-sig-sequencing mailing list, Martin already mentioned it might be due to the missing last line as separator.


    But I do not know how to add a new line after the last line in my file. Does anyone give me some hints?

    Many thanks in advance!
    Wei

  • #2
    You could do it in Perl:

    Code:
    open OUTFILE, ">>file.fastq" or die $!; # open for append
    print OUTFILE "\n";
    close OUTFILE;

    Comment


    • #3
      Thank you so much nodonb! That works! even I do not know the meaning of each code.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Addressing Off-Target Effects in CRISPR Technologies
        by seqadmin






        The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...
        08-27-2024, 04:44 AM
      • seqadmin
        Selecting and Optimizing mRNA Library Preparations
        by seqadmin



        Sequencing mRNA provides a snapshot of cellular activity, allowing researchers to study the dynamics of cellular processes, compare gene expression across different tissue types, and gain insights into the mechanisms of complex diseases. “mRNA’s central role in the dogma of molecular biology makes it a logical and relevant focus for transcriptomic studies,” stated Sebastian Aguilar Pierlé, Ph.D., Application Development Lead at Inorevia. “One of the major hurdles for...
        08-07-2024, 12:11 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 08-27-2024, 04:40 AM
      0 responses
      16 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 08-22-2024, 05:00 AM
      0 responses
      293 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 08-21-2024, 10:49 AM
      0 responses
      135 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 08-19-2024, 05:12 AM
      0 responses
      124 views
      0 likes
      Last Post seqadmin  
      Working...
      X