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  • Normalization with champ.norm in ChAMP Pakcage

    I am trying to use champ.norm function in the Bioconductor package ChAMP for our DNA methylation data for 389 samples.
    When I run champ.norm function I found a problem. At the first time it stopped at the 172th sample, at the second time it stopped at the 53rd sample, at the third time, and the fourth time, and so on, it just stopped at some samples.
    Could any one please tell me what is happening here, and what could be a good solution for this?

  • #2
    Hi,

    You might want to try to disable the plot for BMIQ nomalization.
    champ.norm(plot.BMIQ = FALSE)

    I am also a beginner but it seems to be the first step to try. it worked for me but I sometimes get other errors with the normalization. It is not consistent so I am a bit confused...

    Anyway Hope it helps

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