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  • lwhitmore
    Member
    • Aug 2013
    • 70

    #1

    CutAdapt Statistic output

    Hey,
    I am a first cut adapt user and was alittle confused by the statistic output

    Here is the output
    === Adapter 1 ===

    Adapter 'AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT', length 33, was trimmed 2371637 times.

    No. of allowed errors:
    0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-33 bp: 3

    Overview of removed sequences
    length count expect max.err error counts
    3 195855 121796.5 0 195855
    4 101281 30449.1 0 101281
    5 81153 7612.3 0 81153
    6 75593 1903.1 0 75593
    7 74197 475.8 0 74197
    8 72866 118.9 0 72866
    9 72255 29.7 0 72086 169

    Based on the information provided above the 0-9 bases removed should have an error rate of 0.

    However if you look at the bottom line which says 9 bases are being removed from the read, the max error rate is 0, we still see error of 1 being allowed the 169. Can anyone explain why this might be happening

    Thanks
    Leanne
  • Brian Bushnell
    Super Moderator
    • Jan 2014
    • 2709

    #2
    That's interesting; sounds like a bug, though not a very important one. But if I can make a suggestion, you should probably be using BBDuk instead In my tests, it's much faster and more accurate.

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