@Brian Bushnell
Ah OK, I see. So it "expects" to not find the adapter sequence there, since it has hopefully been removed by BBDuk. Slightly unrelated, I am using RNA-Seq data for a coniferous tree species, and am assembling using conventional assemblers such as Trinity, Velvet-Oases, etc. BBMerge is appropriate for this purpose, right? I keep noticing a lot of threads talking about 16S data, or amplicon data, and I haven't even heard of the assemblers that you mentioned
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Thanks!
Ah OK, I see. So it "expects" to not find the adapter sequence there, since it has hopefully been removed by BBDuk. Slightly unrelated, I am using RNA-Seq data for a coniferous tree species, and am assembling using conventional assemblers such as Trinity, Velvet-Oases, etc. BBMerge is appropriate for this purpose, right? I keep noticing a lot of threads talking about 16S data, or amplicon data, and I haven't even heard of the assemblers that you mentioned
![Big Grin](https://www.seqanswers.com/core/images/smilies/biggrin.png)
Thanks!
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