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  • migrau
    Junior Member
    • Jun 2014
    • 4

    #1

    pacBioToCA 0 split bases

    Hi forum,
    I am using PBcR, following the wiki tutorial, "Correction Using Complementary High-Identity Sequences"
    When I run pacBioToca to generate corrected pacbio sequences, the process stops suddenly:

    <Linux-amd64>/bin/fastqToCA -libraryname illumina -technology illumina -type sanger -innie -mates illumina_forward_paired.fastq,illumina_reverse_paired.fastq > illumina.frg

    <Linux-amd64>/bin/pacBioToCA -length 500 -partitions 200 -l pacbio -t 8 -s pacbio.spec -fastq pacbio.filtered_subreads.fastq illumina.frg > run.out 2>&1


    The last log lines are (without previous error messages):

    cp asm.layout.err /apps/wgs-8.1/DATA//pacbio.correction.err
    Total split bases is 0 vs 9188565 so ratio is 0
    ----------------------------------------END Mon Jun 9 18:29:42 2014 (0 seconds)
    ----------------------------------------START Mon Jun 9 18:29:42 2014
    cp asm.layout.hist /apps/wgs-8.1/DATA//pacbio.correction.hist
    ----------------------------------------END Mon Jun 9 18:29:42 2014 (0 seconds)
    ----------------------------------------START Mon Jun 9 18:29:42 2014
    cat `ls [0-9]*.fasta |grep trim |sort -T . -rnk1` > /apps/wgs-8.1/DATA//pacbio.fasta

    I am not sure which parameter I have to edit and why the total split base are 0.
    CA 8.1 is correctly installed, if I use the sample example data from the tutorial, it works.

    Thanks!
  • migrau
    Junior Member
    • Jun 2014
    • 4

    #2
    Solved. It was a problem with my input pacbio data. The coverage was around 20% so there were not enougth overlapped reads.
    Run:
    samtools mpileup -uf pacbio.fastq illumina.bam | bcftools view -cg - | vcfutils.pl vcf2fq > out.fq
    And check the coverage from out.fq

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