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  • Need script to estimate error rate

    Hi, does anyone has the script to estimate the following for Illumina reads? Care to share?
    a) error rate
    b) GC content
    c) Phred score distribution across the read length

    Thanks,
    Melissa

  • #2
    Hi Melissa

    have a look at my htseq-qa script: http://www-huber.embl.de/users/anders/HTSeq/doc/qa.html

    Is this what you are looking for?

    Cheers
    Simon

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