Tweet
I just started using FASTX to trim my Illumina reads (synthetic long reads with Sanger encoding). I used the fastq_quality_trimmer command (command below) and it ran with no issues.
./fastq_quality_trimmer -Q33 -t 28 -i /LR6000035-DNA_A01-LRAAD-01_LongRead.fastq -o L1_trimmed_28.fastq
As a sanity check, I am want to run the fastx quality stats on my trimmed reads but I keep getting an error message 'Segmentation default'. Below is the command that I am using:
./fastx_quality_stats -Q33 -N -i L1_trimmed_28.fastq -o L1_trimmed_28_stats
Does anyone have any idea as to what is going on? I am using the latest version of fastx and am also specifying the -Q parameter.
Thank you very much!
./fastq_quality_trimmer -Q33 -t 28 -i /LR6000035-DNA_A01-LRAAD-01_LongRead.fastq -o L1_trimmed_28.fastq
As a sanity check, I am want to run the fastx quality stats on my trimmed reads but I keep getting an error message 'Segmentation default'. Below is the command that I am using:
./fastx_quality_stats -Q33 -N -i L1_trimmed_28.fastq -o L1_trimmed_28_stats
Does anyone have any idea as to what is going on? I am using the latest version of fastx and am also specifying the -Q parameter.
Thank you very much!
Comment