Hello,
Library specs: Paired End, Read length 150 bp, V3 region 16S rRNA gene
Platform : MiSeq, Illumina
Experiment : Wheat Field, rhizosphere samples, Elevated CO2 and temperature
Computational platform : AWS EC2, Qiime 1.8.0
I am a Qiime newbie, have total 39 (13x3) samples, which represent 12 Treatments and 1 control with 3 replicates per Treatment and also control.
According to Qiime Documentation , for creating the metadata file I require Sample ID, Barcode, Primer sequence and description.
As this sequencing was done by a commercial provider, they refuse to provide barcode sequences.
Ques1: What should I use as Sample ID ? Does it have to be a part of read name?
Ques2: For Beta diversity analysis, I would like the 3 replicates pooled for every treatment, how should the mapping file be constructed for this?
Given that I do not have barcode sequence.
Any help / pointers / comments are appreciated.
--
pg
Library specs: Paired End, Read length 150 bp, V3 region 16S rRNA gene
Platform : MiSeq, Illumina
Experiment : Wheat Field, rhizosphere samples, Elevated CO2 and temperature
Computational platform : AWS EC2, Qiime 1.8.0
I am a Qiime newbie, have total 39 (13x3) samples, which represent 12 Treatments and 1 control with 3 replicates per Treatment and also control.
According to Qiime Documentation , for creating the metadata file I require Sample ID, Barcode, Primer sequence and description.
As this sequencing was done by a commercial provider, they refuse to provide barcode sequences.
Ques1: What should I use as Sample ID ? Does it have to be a part of read name?
Ques2: For Beta diversity analysis, I would like the 3 replicates pooled for every treatment, how should the mapping file be constructed for this?
Given that I do not have barcode sequence.
Any help / pointers / comments are appreciated.
--
pg
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