Hi!
I'm trying to align my data using Tophat2 and the following files:
genome: Homo_sapiens.GRCh37.75.dna.primary_assembly.fa (from which index files were built using Bowtie)
gtf file: gencode.v19.2wayconspseudos
I'v also tried to align using UCSC available human genome. But, in both cases no "accepted_hits.bam" is generated. Instead of, two folders, named "logs" and "tmp", and a file "prep_reads.info".
Has anybody already had this problem?
Thaks
I'm trying to align my data using Tophat2 and the following files:
genome: Homo_sapiens.GRCh37.75.dna.primary_assembly.fa (from which index files were built using Bowtie)
gtf file: gencode.v19.2wayconspseudos
I'v also tried to align using UCSC available human genome. But, in both cases no "accepted_hits.bam" is generated. Instead of, two folders, named "logs" and "tmp", and a file "prep_reads.info".
Has anybody already had this problem?
Thaks
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