I am trying to determine the method used to generate my RNAseq data. On the Illumina website, the read length are either 1*36bp or 2*50bp. My fastq reads are non-strand-specific and non-paired sequences of 50bp each. I'm not sure how to interpret this...
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Illumina has various kits for RNAseq library prep. As Brian has pointed, libraries from all kits can be sequenced for various length and also as single read or paired read. Core centre or person preparing libraries should be able to provide information on kit and method used for library prep.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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