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  • chariko
    Member
    • Jun 2010
    • 56

    #1

    Understanding Mauve Contig MOver Alignment Result

    Hi,
    I have used Mauve Contig mover to reorder 20 contigs into just one using a reference genome file. When running Mauve Contig Mover, I obtain 2 alignment folders and when I look through the Alignment2 file I obtain various files. The one I do not understand is the one called alignment. It looks like:

    #FormatVersion Mauve1
    #Sequence1File reference.gb
    #Sequence1Format GenBank
    #Annotation1File reference.gb
    #Annotation1Format GenBank
    #Sequence2File 20_contigs.fasta
    #Sequence2Format FastA
    #BackboneFile alignment2.bbcols

    > 1:1-46592 + ..alignment2/reference.gb
    acgtgcc--tcg....
    > 2:643-45360 +alignment2/20_contigs.fasta
    ttt---cgttaacca...
    > 1:46633-47274 + ../alignment2/reference.gb
    tgtacga
    > 2:1-642 + alignment2/20_contigs.fasta
    cgggc
    > 1:46593-46632 + ../alignment2/reference.gb
    cgggc
    > 2:45361-47649 + alignment2/20_contigs.fasta


    Which one is my unique contig? the ones whose fasta header ended in reference or in 20_contigs?

    Any suggestion is really appreciated.

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