It says that tblastn is more likely to find distantly related sequences than the blast, blasts, and blastp. I wonder why is it? I generally use Blastx.
By definition, tblastn compares query protein sequence with the database after translating each nucleotide sequence into protein using all six reading frames
whereas
Blastx first translates query sequence into amino acids in six reading frames (three forward and three back) then compares the protein sequences with protein databases.
Any insight would be greatly appreciated!
Thanks.
By definition, tblastn compares query protein sequence with the database after translating each nucleotide sequence into protein using all six reading frames
whereas
Blastx first translates query sequence into amino acids in six reading frames (three forward and three back) then compares the protein sequences with protein databases.
Any insight would be greatly appreciated!
Thanks.
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