Hi everyone.
I'm have a question…, very basic I'm afraid.
I just received the results of my RNA-seq sequencing reaction, mate-pair, and it seems that there was a problem during read 2 sequencing. They propose me to start analyzing reads 1 while waiting for the repetition of the repeat the run.
I don't really like this procedure. I would be introducing a huge bias right? Is there any normalization able to get rid of such a bias?
Thank you in advance for your comments.
Cristina
I'm have a question…, very basic I'm afraid.
I just received the results of my RNA-seq sequencing reaction, mate-pair, and it seems that there was a problem during read 2 sequencing. They propose me to start analyzing reads 1 while waiting for the repetition of the repeat the run.
I don't really like this procedure. I would be introducing a huge bias right? Is there any normalization able to get rid of such a bias?
Thank you in advance for your comments.
Cristina
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