Hello,
I am working with miRNA for quite some time now and use miRDeep2 for it. I have these two queries to which i've searched a lot for the answer but without any success.
1. the read depths for miRBase hits(both result.csv & expression.csv) doesn't equal to total reads of the input file. I suppose it should be like Total reads= miRBase hits + novel miRNAs isn't it??!!.....or am i going wrong
2. what is the last part of result.csv signifies ??!!
my pipeline: raw reads-> cutadapt -> rRNA filtering -> mapping to genome -> miRDeep2
Any help in this regard will be highly appreciated...thanks in advanc !!!
I am working with miRNA for quite some time now and use miRDeep2 for it. I have these two queries to which i've searched a lot for the answer but without any success.
1. the read depths for miRBase hits(both result.csv & expression.csv) doesn't equal to total reads of the input file. I suppose it should be like Total reads= miRBase hits + novel miRNAs isn't it??!!.....or am i going wrong
2. what is the last part of result.csv signifies ??!!
my pipeline: raw reads-> cutadapt -> rRNA filtering -> mapping to genome -> miRDeep2
Any help in this regard will be highly appreciated...thanks in advanc !!!