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  • colaneri
    Member
    • Jul 2012
    • 30

    #1

    summarizeOverlaps error

    Hello,
    I'm following the RNA-seq workflow for differential gene expression
    white paper by Michael Love Simon Anders and Wolfgang Huber.

    This is the code
    Code:
    ### read the table: sampleTable.csv
    
    sampleTable <- read.csv("sampleTable.csv", header=TRUE);
    
    ### build the full path to the tophat produced bam files
    
    bamFiles <- file.path(".", sampleTable$dirName, sampleTable$fileName);
    
    ### see the created vector with paths
    
    bamFiles
    
    ##### Use the BamFile function from the RsamTools to se if these paths are functional
    
    library ("Rsamtools");
    seqinfo(BamFile(bamFiles[1]));
    
    #Counting reads in genes
    
    library("GenomicFeatures");
    
    hse <-makeTranscriptDbFromGFF("/proj/seq/data/TAIR10_Ensembl/Annotation/Genes/genes.gtf", format="gtf")
    exonsByGene <- exonsBy(hse, by="gene");
    
    ## Use the function summarizeOverlaps to count reads in the gene
    library("GenomicAlignments")
    se <- summarizeOverlaps(exonsByGene, BamFileList(bamFiles), mode="Union", singleEnd=TRUE, ignore.strand=FALSE, fragments=FALSE);

    however I got this error and I have not idea how to fix it:

    Error in .summarizeOverlaps_BamFileList(features, reads, mode, ignore.strand = ignore.strand, :
    duplicate 'names(reads)' not allowed


    Can someone help please!!

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