Hello all,
I realize that this is already a frequently asked question. Nevertheless, the latest posts I could find on this forum are already 1-2 years old!
What software would be recommended to detect fusions in RNA-seq data? At first thought, we would go for FusionCatcher, deFuse and/or Tophat-fusion. Any experience or comparisons for these tools?
Also, any suggestions on the amount of coverage/reads necessary to detect fusions. We would go for 100bp paired-end for sure, but not sure how deep to sequence.
Many thanks for any thoughts on this,
Lien
I realize that this is already a frequently asked question. Nevertheless, the latest posts I could find on this forum are already 1-2 years old!
What software would be recommended to detect fusions in RNA-seq data? At first thought, we would go for FusionCatcher, deFuse and/or Tophat-fusion. Any experience or comparisons for these tools?
Also, any suggestions on the amount of coverage/reads necessary to detect fusions. We would go for 100bp paired-end for sure, but not sure how deep to sequence.
Many thanks for any thoughts on this,
Lien
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