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  • hellsingwyk
    replied
    Thank you very much!!!
    Let me try try it first~

    Leave a comment:


  • GenoMax
    replied
    If you used progressiveMauve to generate the alignments then there may be (.xmfa) format alignment files in the directory you did the work in. If they are not there you may have to run mauve on the command line (http://darlinglab.org/mauve/user-gui...sivemauve.html).

    Once you have the alignment files you can use this script http://www.bioperl.org/wiki/Converting_alignment_files (latest versions is supposed to be here: https://github.com/lskatz/lskScripts to convert .xmfa files to fasta.

    Leave a comment:


  • hellsingwyk
    replied
    Dear all,

    I really have no idea on it and now I only have reordered contigs and have no idea on draft genome development.........

    Could anyone give me a hand??

    Leave a comment:


  • How to get complete consensus genome from ordered contigs developed from Mauve?

    Dear all,

    I am working on de novo assembly in order to get the whole target genome sequence for phylogenetic analysis.

    And now I successfully run velvet to obtain contigs and reorder those contigs using Mauve ---- a java based GUI tools with the help of reference sequence.

    However, I have no idea on retrieving complete target genome sequence (consensus sequence). Could anyone give me a hand?

    Thank you very much for your kind attention!!!

    Best Regards
    Michael

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    New and advanced multiomics tools and technologies have opened new avenues of research and markedly enhanced various disciplines such as disease research and precision medicine1. The practice of merging diverse data from various ‘omes increasingly provides a more holistic understanding of biological systems. As Maddison Masaeli, Co-Founder and CEO at Deepcell, aptly noted, “You can't explain biology in its complex form with one modality.”

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