Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • zhanxw
    Junior Member
    • Jun 2009
    • 7

    #1

    BFAST index, not understand warnings

    Hello,

    I want to created index files in color space from NCBI build 36 in color space.
    Here is my command
    `which time` bfast index -f NCBI36.fa -n 8 -w 14 -i 1 -A 1 -m 1111111111111111111111

    Then I got warning like "startContig was less than zero.", "endContig was greater than the number of contigs in the reference genome.". But I am sure I am using the same FASTA reference genome file. So is there anything I should do for these warnings? And is anyone have experience about how long it takes to build this index file?

    Part of the outputs:
    ------------------------------------------------------
    Checking input parameters supplied by the user ...
    Validating fastaFileName NCBI36.fa.
    Validating tmpDir path ./.
    Input arguments look good!
    ************************************************************
    ************************************************************
    Printing Program Parameters:
    programMode: [ExecuteProgram]
    fastaFileName: NCBI36.fa
    space: [Color Space]
    mask: 1111111111111111111111
    depth: 0
    hashWidth: 14
    indexNumber: 1
    repeatMasker: [Not Using]
    startContig: 0
    startPos: 0
    endContig: 2147483647
    endPos: 2147483647
    exonsFileName: [Not Using]
    numThreads: 8
    tmpDir: ./
    timing: [Not Using]
    ************************************************************
    ************************************************************
    Reading in reference genome from NCBI36.fa.cs.brg.
    In total read 23 contigs for a total of 3022646526 bases
    ************************************************************
    Creating the index...
    ************************************************************
    Warning: startContig was less than zero.
    Defaulting to contig=1 and position=1.
    ************************************************************
    ************************************************************
    Warning: endContig was greater than the number of contigs in the reference genome.
    Defaulting to reference genome's end contig=23 and position=154913754.
    ************************************************************
    Currently on [contig,pos]:
    [-------4,---27000000]

    ------------------------------------------------------
  • nilshomer
    Nils Homer
    • Nov 2008
    • 1283

    #2
    Originally posted by zhanxw View Post
    Hello,

    I want to created index files in color space from NCBI build 36 in color space.
    Here is my command
    `which time` bfast index -f NCBI36.fa -n 8 -w 14 -i 1 -A 1 -m 1111111111111111111111

    Then I got warning like "startContig was less than zero.", "endContig was greater than the number of contigs in the reference genome.". But I am sure I am using the same FASTA reference genome file. So is there anything I should do for these warnings? And is anyone have experience about how long it takes to build this index file?

    Part of the outputs:
    ------------------------------------------------------
    Checking input parameters supplied by the user ...
    Validating fastaFileName NCBI36.fa.
    Validating tmpDir path ./.
    Input arguments look good!
    ************************************************************
    ************************************************************
    Printing Program Parameters:
    programMode: [ExecuteProgram]
    fastaFileName: NCBI36.fa
    space: [Color Space]
    mask: 1111111111111111111111
    depth: 0
    hashWidth: 14
    indexNumber: 1
    repeatMasker: [Not Using]
    startContig: 0
    startPos: 0
    endContig: 2147483647
    endPos: 2147483647
    exonsFileName: [Not Using]
    numThreads: 8
    tmpDir: ./
    timing: [Not Using]
    ************************************************************
    ************************************************************
    Reading in reference genome from NCBI36.fa.cs.brg.
    In total read 23 contigs for a total of 3022646526 bases
    ************************************************************
    Creating the index...
    ************************************************************
    Warning: startContig was less than zero.
    Defaulting to contig=1 and position=1.
    ************************************************************
    ************************************************************
    Warning: endContig was greater than the number of contigs in the reference genome.
    Defaulting to reference genome's end contig=23 and position=154913754.
    ************************************************************
    Currently on [contig,pos]:
    [-------4,---27000000]

    ------------------------------------------------------
    You can ignore the warnings if you didn't specify the "-s" or "-e" options. Each index takes about 4-5 hours to build (8 threads).

    Comment

    Latest Articles

    Collapse

    • kinpict.com
      Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by kinpict.com
      The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
      Today, 06:37 PM
    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2





      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
      10-30-2026, 06:46 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 11-12-2026, 07:59 AM
    0 responses
    27 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 11-05-2026, 09:25 AM
    0 responses
    35 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 11-03-2026, 11:54 AM
    2 responses
    56 views
    0 reactions
    Last Post kinpict.com  
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    175 views
    0 reactions
    Last Post SEQadmin2  
    Working...