strand bias?
I have 3 lanes of of PE bisulf-seq data that I've aligned via bismark. I've merged the data and converted it to a UCSC track to visualize coverage. We've noticed what appears to be regions of significant strand bias randomly throughout the genome, with 1kb+ regions that have (for example) 5x coverage of reads mapped to the + strand and 40x coverage of reads mapped to the - strand.
Has anyone else noticed behavior like this? Any ideas on the source?
Thanks!
I have 3 lanes of of PE bisulf-seq data that I've aligned via bismark. I've merged the data and converted it to a UCSC track to visualize coverage. We've noticed what appears to be regions of significant strand bias randomly throughout the genome, with 1kb+ regions that have (for example) 5x coverage of reads mapped to the + strand and 40x coverage of reads mapped to the - strand.
Has anyone else noticed behavior like this? Any ideas on the source?
Thanks!
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