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  • idedios
    Member
    • Mar 2014
    • 20

    #1

    Problem replacing contigs in a dbsnp vcf using Picard SortVcf

    Right now I'm trying to change the contigs in the dbsnp vcf to match those of my reference genome (from 1, 2, ..., Y, MT to chrM, chr1, chr2,...)

    I'm currently using JDK 1.7 u79 for compatibility with MuTect 1.7

    SortVcf was used as such:

    "java -jar picard.jar SortVcf \
    INPUT=dbsnp.vcf \
    OUTPUT=dbsnp.fixed.vcf \
    SEQUENCE_DICTIONARY=hg19.dict

    Here's SortVcf's output:

    Exception in thread "main" java.lang.NullPointerException
    at htsjdk.variant.variantcontext.VariantContextComparator.compare(VariantContextComparator.java:84)
    at htsjdk.variant.variantcontext.VariantContextComparator.compare(VariantContextComparator.java:21)
    at java.util.TimSort.countRunAndMakeAscending(TimSort.java:324)
    at java.util.TimSort.sort(TimSort.java:203)
    at java.util.Arrays.sort(Arrays.java:727)
    at htsjdk.samtools.util.SortingCollection.spillToDisk(SortingCollection.java:218)
    at htsjdk.samtools.util.SortingCollection.add(SortingCollection.java:165)
    at picard.vcf.SortVcf.sortInputs(SortVcf.java:154)
    at picard.vcf.SortVcf.doWork(SortVcf.java:87)
    at picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:187)
    at picard.cmdline.PicardCommandLine.instanceMain(PicardCommandLine.java:95)
    at picard.cmdline.PicardCommandLine.main(PicardCommandLine.java:105)

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