Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • nimrod337
    replied
    Unfortunately, I started on the older samtools, and upgraded to 1.2 to see if that fixed the problem. It did not. I have cleared up some disk space, and rebooted to clear the RAM. Cross your fingers!!

    Leave a comment:


  • GenoMax
    replied
    That header looks good. Is it possible for you to try the older version of samtools (0.1.19) for the sort? If you are certain ample disk space is available then this may be worth a try.

    Leave a comment:


  • nimrod337
    replied
    samtools view -H looks like this:

    @SQ SN:scaffold_829 LN:219473
    @SQ SN:scaffold_4279 LN:55059
    @PG ID:bwa PN:bwa VN:0.7.5a-r405

    (the scaffold_###) is the fasta header in my reference

    How could I check if I am running out of resources? Does anything like that get printed in the log file somewhere? I re-ran the sort with -m 8GB to ensure that it wouldn't run out of RAM (16 on the machine) but I got this error:

    [E::hts_open] fail to open file 'M6scaff_BWA.bam.sorted.0001.bam'

    The BAM is 25G

    samtools v 1.2

    Thanks for the help. Anything helps at this point.

    Edit: I can see the temporary M6scaff_BWA.bam.sorted.####.bam files in the folder, Why then, would it have trouble opening it?
    Last edited by nimrod337; 04-20-2015, 11:09 AM. Reason: New info

    Leave a comment:


  • GenoMax
    replied
    Does the header on your BAM look right (when you do samtools view on it)? Are you running out of resources anywhere (/tmp, disk, memory etc) in this process? What is the size of the BAM file? What version of samtools are you using?

    Leave a comment:


  • samtools sort [bam_merge_core] [bam_sort_core] errors?

    Okay, I've run out of ideas. I cannot get this BAM file to sort. I have a reference sequence in FASTA format that has 2 'Chromosomes' that are actually just scaffolds from a genome that isn't finished yet. The first time I ran my illumina reads through the workflow (BWA>SAM>BAM>sort>index... etc) Then when I tried to view it, it failed because there were replications in the BAM headers. I discovered that these were my fault from the python script I wrote to pull out the FASTA scaffolds that matched the gene I was looking for in the pile of scaffolds. Anyway: I fixed the script, and got the new reference, that is the same as the old one, but without the repetitive '>header' accidentally added 11 times. I ran BWA, no problem. I converted the SAM to BAM with samtools, no problem. But now I am trying to sort and index the BAM so I can look at it an whatnot, and the command I am running is:

    samtools sort M6scaff_BWA.bam M6scaff_BWA.bam.sorted

    the error I get is:

    [bam_sort_core] merging from 142 files...
    [W::bam_hdr_read] EOF marker is absent. The input is probably truncated.
    [E::hts_open] fail to open file 'M6scaff_BWA.sorted.0118.bam'
    [bam_merge_core] fail to open file M6scaff_BWA.sorted.0118.bam

    I have looked around the interwebs for a solution, and most of the people that have this error are using the -f argument in samtool sort, and when they remove it, it's fixed. I am not using the -f argument, so I have no recourse. Can someone please suggest something to try? I am banging my head against a wall for a whole weekend now, and I just don't know what to do.

    Thanks so much

Latest Articles

Collapse

  • seqadmin
    Multiomics Techniques Advancing Disease Research
    by seqadmin


    New and advanced multiomics tools and technologies have opened new avenues of research and markedly enhanced various disciplines such as disease research and precision medicine1. The practice of merging diverse data from various ‘omes increasingly provides a more holistic understanding of biological systems. As Maddison Masaeli, Co-Founder and CEO at Deepcell, aptly noted, “You can't explain biology in its complex form with one modality.”

    A major leap in the field has
    ...
    02-08-2024, 06:33 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 02-23-2024, 04:11 PM
1 response
22 views
0 likes
Last Post kim897
by kim897
 
Started by seqadmin, 02-21-2024, 08:52 AM
0 responses
39 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-20-2024, 08:57 AM
0 responses
26 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-14-2024, 09:19 AM
0 responses
58 views
0 likes
Last Post seqadmin  
Working...
X