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  • Trinity genome guided error

    Hello,
    Getting this error after running for many hrs:
    Please anybody got into same problem?

    #############################################################################
    This was the command:

    Trinity --genome_guided_bam accepted_hits.sorted.bam --genome_guided_max_intron 10000 --max_memory 60G --CPU 30
    ##############################################################################


    ----------------------------------------------------------------------------------
    -------------- Trinity Phase 1: Clustering of RNA-Seq Reads ---------------------
    ----------------------------------------------------------------------------------

    Wednesday, April 22, 2015: 16:51:14 CMD: find Dir_* -name '*reads' > read_files.list
    Wednesday, April 22, 2015: 16:51:14 CMD: /home/usr/bin/trinityrnaseq-2.0.6/util/support_scripts/GG_write_trinity_cmds.pl --reads_list_file read_files.list --CPU 1 --max_memory 1G --full_cleanup --seqType fa --trinity_complete > trinity_GG.cmds

    ####################################################################################
    #
    # usage: /home/usr/bin/trinityrnaseq-2.0.6/util/support_scripts/GG_write_trinity_cmds.pl --reads_list_file <string> [Trinity params]
    #
    # Required:
    #
    # --reads_list_file <string> file containing list of filenames corresponding
    # to the reads.fasta
    #
    #
    #####################################################################################


    Error, cmd: /home/usr/bin/trinityrnaseq-2.0.6/util/support_scripts/GG_write_trinity_cmds.pl --reads_list_file read_files.list --CPU 1 --max_memory 1G --full_cleanup --seqType fa --trinity_complete > trinity_GG.cmds died with ret 65280 at /home/usr/bin/trinityrnaseq-2.0.6/Trinity line 2116.


    Thanks
    Sandesh

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