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Hayden Waller · 03-03-2022, 10:55 AM

Hi all,

The project I am currently working on involves five closely related cricket species in the same genus. I have 8 (neutral) replicates...

remivarney · 11-06-2018, 10:01 AM

Apologies for what is an often repeated topic, but with (I believe) a new twist.

I have several RNA libraries that I amplified with the...

illuminaGA · 05-21-2018, 06:57 PM

Dear All, i am working on a project that will do De novo RNA-Seq Assembly using Trinity. Totally have 16 samples from 2 projects.

1. 8...

Physalia-courses · 04-30-2018, 01:15 AM

Dear all,

we will run a Trinity Workshop with Brian and Nicolas Delhomme in Berlin this June (11-15).

Sign up if interested!...

roussine · 01-25-2018, 08:39 AM

Hello!
can someone please give an opinion: does that make sense to merge PE reads before a de novo RNA-seq assembly (say with Trinity)?
...

nurgling · 04-08-2017, 12:02 PM

Hi there,

I have some questions regarding CLC Bio vs. Trinity for de novo RNA seq assembly. When I was told how to process my data by a postdoc...

jmah · 03-15-2017, 03:18 PM

I am new to Seqanswers (Hi!) but so far have not yet found a helpful thread for my particular problem.

I am trying to do two de novo assemblies...

gwilymh · 02-19-2016, 12:21 PM

Hi Trinity community,

I am working on step 6. Running RNAMMER to identify rRNA transcripts of the Trinoate protocol detailed on https://...

Xiaopei · 01-19-2016, 02:14 AM

Hi,

I am assembling about 540 million single RNA sequences with Trinity. I used normalized setting to finish it sooner.

...

peromhc · 01-08-2016, 06:08 AM

Hi All,

I wanted to make sure people have seen the new Oyster River Protocol for transcriptome assembly. In brief, it’s an evidence...

huan · 12-20-2015, 10:33 PM

Hi,
I have done the De novo RNA-Seq Assembly using Trinity with my fish data, but the number of unigenes I get is 333,843, and the N50 Length is...

Mocca · 10-08-2015, 03:52 AM

Hi everyone,

For a while now I have been working on my de novo Trinity assembly (fish species). Using my de novo transcriptome I have...

gwilymh · 09-05-2015, 03:54 PM

I am trying to install trinity v2.0.6 on a Linux cluster, and am getting a strange error message after running ./configure and then make:

make[1]:

...

GillermoPonz · 05-28-2015, 08:19 AM

Hi everyone,

I'm about to perform a transcriptome assembly with Trinity, but I have a basic question.

I have two RNA-Seq...

gwilymh · 05-13-2015, 01:56 PM

I am currently using trinity v2.6.0. My jobs keep crashing at the butterfly stage of the analysis, with such error messages as:
succeeded(1008),...

Differential Expression analyses between species