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  • mcrusch
    Junior Member
    • Mar 2011
    • 6

    Database schema/data model for sequencing methods

    Does anybody know about a database, database schema, data model, etc., for modeling information about next gen sequencing methods or experiments?

    We do most of our tracking at the level of a "sample" and a "sequencing type", but a sample can have multiple libraries that have each been prepped in different ways (e.g. different fragment lengths). For sequencing type, we might call it "RNA-Seq", but is it total stranded, poly-A selected, etc.? There is a lot more complexity here than what we currently track, and as we expand out, I don't want to re-invent the wheel.

    Anybody know of any existing solutions? I could make use of anything from a full featured product down to an E-R diagram.

    Thanks,
    Michael

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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.


    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM
  • SEQadmin2
    From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
    by SEQadmin2


    Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
    ...
    06-02-2026, 10:05 AM

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