Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • bjackson
    Junior Member
    • May 2015
    • 6

    #1

    CummeRbund shows 95% NAs for gene lists

    Code:
    cuff_data = readCufflinks(dir=paste0(getwd(),"/../cuffdiff/"), 
                              gtfFile = paste0(getwd(),"/../cuffmerge/merged.gtf"), 
                              genome="hg19", rebuild=F)
    
    #get significant IDs
    diffGeneIDs = getSig(cuff_data, level="genes", alpha=0.05)
    
    diffGenes = getGenes(cuff_data, diffGeneIDs)
    
    featureNames(diffGenes)[1:40,]
    tracking_id gene_short_name
    1 XLOC_000265 CROCC
    2 XLOC_000328 ALPL
    3 XLOC_000443 SYTL1
    4 XLOC_000679 ARTN
    5 XLOC_000938 RNU6-387P
    6 XLOC_001003 FAM73A,RNA5SP21
    7 XLOC_001244 GSTM1,GSTM2
    8 XLOC_001530 ADAMTSL4,AL356356.1,MIR4257
    9 XLOC_001735 FCER1A
    10 XLOC_001988 AXDND1
    11 XLOC_002089 RGS1
    12 XLOC_002211 NFASC,RP11-494K3.2
    13 XLOC_002282 HHAT,KCNH1
    14 XLOC_002522 RYR2
    15 XLOC_002961 PLA2G2A
    16 XLOC_003294 SLC2A1
    17 XLOC_003772 MIR137HG
    18 XLOC_004252 ASH1L,ASH1L-IT1,MIR555
    19 XLOC_004467 RP1-117P20.3,SELE
    20 XLOC_004799 CR1L
    21 XLOC_004802 CD34
    22 XLOC_004843 <NA>
    23 XLOC_005255 <NA>
    24 XLOC_005256 <NA>
    25 XLOC_005257 <NA>
    26 XLOC_005263 <NA>
    27 XLOC_005264 <NA>
    28 XLOC_005265 <NA>
    29 XLOC_005273 <NA>
    30 XLOC_005276 <NA>
    31 XLOC_005277 <NA>
    32 XLOC_005288 <NA>
    33 XLOC_005291 <NA>
    34 XLOC_005292 <NA>
    35 XLOC_005293 <NA>
    36 XLOC_005294 <NA>
    37 XLOC_005322 <NA>
    38 XLOC_005331 <NA>
    39 XLOC_005332 <NA>
    40 XLOC_005333 <NA>
    41 XLOC_005336 <NA>

    Code:
    mean(is.na(as.vector(featureNames(diffGenes)[2])))
    [1] 0.9691279

    This is human data and I have used the same GTF / genome data throughout, so why are so few genes labeled? Only about 3% of the total 6284 differentially expressed genes have labels.
    I have followed the protocol in the nature paper (tophat -> cufflinks -> cuffmerge -> cuffdiff)

    Thanks
    Last edited by bjackson; 05-11-2015, 05:55 PM.
  • bjackson
    Junior Member
    • May 2015
    • 6

    #2
    Still looking through this data set. I have 194 named differentially expressed genes. What is the 'normal' amount of differentially expressed genes that you would expect? I know it can vary a lot based on how different the conditions are, but is this in the range of possibility? A lot of the unnamed bits are small chunks or exons. Does this sound like it might be a true result instead of an error in annotation?

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Yesterday, 11:13 AM
    0 responses
    21 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, Yesterday, 10:48 AM
    0 responses
    18 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    36 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    42 views
    0 reactions
    Last Post SEQadmin2  
    Working...