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  • SSPACE scaffolding

    Hi All,

    I am using SSPACE for scaffolding. After running sspace i am getting same no of scaffolds as the no of contigs i got but the no of scaffold should be less then the no of contig. Am i correct?

    Here is my command

    perl SSPACE_Standard_v3.0.pl -l libraries.txt -s contigs.fa -T 4 -b out

    The summary file

    READING READS lib1:
    ------------------------------------------------------------
    Total inserted pairs = 2880237
    ------------------------------------------------------------



    LIBRARY lib1 STATS:
    ################################################################################

    MAPPING READS TO CONTIGS:
    ------------------------------------------------------------
    Number of single reads found on contigs = 209
    Number of read-pairs used for pairing contigs / total pairs = 33 / 33
    ------------------------------------------------------------

    READ PAIRS STATS:
    Assembled pairs: 33 (66 sequences)
    Satisfied in distance/logic within contigs (i.e. -> <-, distance on target: 3000 +/-750): 0
    Unsatisfied in distance within contigs (i.e. distance out-of-bounds): 0
    Unsatisfied pairing logic within contigs (i.e. illogical pairing ->->, <-<- or <-->): 0
    ---
    Satisfied in distance/logic within a given contig pair (pre-scaffold): 0
    Unsatisfied in distance within a given contig pair (i.e. calculated distances out-of-bounds): 33
    ---
    Total satisfied: 0 unsatisfied: 33


    Estimated insert size statistics (based on 0 pairs):
    Mean insert size = 0
    Median insert size = 0

    REPEATS:
    Number of repeated edges = 0
    ------------------------------------------------------------

    ################################################################################

    SUMMARY:
    ------------------------------------------------------------
    Inserted contig file;
    Total number of contigs = 1058
    Sum (bp) = 35688531
    Total number of N's = 0
    Sum (bp) no N's = 35688531
    GC Content = 45.29%
    Max contig size = 382053
    Min contig size = 300
    Average contig size = 33732
    N25 = 162114
    N50 = 88961
    N75 = 48154

    After scaffolding lib1:
    Total number of scaffolds = 1058
    Sum (bp) = 35688531
    Total number of N's = 0
    Sum (bp) no N's = 35688531
    GC Content = 45.39%
    Max scaffold size = 382053
    Min scaffold size = 300
    Average scaffold size = 33732
    N25 = 162114
    N50 = 88961
    N75 = 48154

    ------------------------------------------------------------

    Can anyone help me to understand why it is coming like this?

    Any help will be appreciate..

    Thanks in advance....

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