Hello, I want to blast a fasta file which the output of usearch after sequence merging and filtering.
I have downloaded all the nucleotide files from NCBI in FASTA format and after inzipping I ended up with a file named just nt. I used the makeblastdb command and got 22(!) .nhr .nin and .nsq files. When I run the blastn command like this, while being in the database directory:
blastn -db /home/database -query /home/COIS/19-3.fa -out outfile.txt
I get an error message saying "No alias or index file found for nucleotide database"
Any ideas on how to fix this?
I have downloaded all the nucleotide files from NCBI in FASTA format and after inzipping I ended up with a file named just nt. I used the makeblastdb command and got 22(!) .nhr .nin and .nsq files. When I run the blastn command like this, while being in the database directory:
blastn -db /home/database -query /home/COIS/19-3.fa -out outfile.txt
I get an error message saying "No alias or index file found for nucleotide database"
Any ideas on how to fix this?
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