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  • Genome not sequence typed

    Hi Members,

    I'm dealing with e. coli genome. I did de novo assembly using SPAdes 3.5, genome size is reasonable ~4.8+mbs.
    Everything is sequenced, including plasmids of the bacteria.

    However, when I try to find their Sequence type I'm finding that they aren't sequence typed.

    I used SRST tool and in-house BLAST pipeline in order to check isolates' sequence type. Both these pipeline fail to identify isolates' sequence type information.
    Used profile of E. coli#1 from pub mlst database.

    I was wondering, what this could mean biologically about the data/genome?
    Bioinformaticscally calm

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