Hi Members,
I'm dealing with e. coli genome. I did de novo assembly using SPAdes 3.5, genome size is reasonable ~4.8+mbs.
Everything is sequenced, including plasmids of the bacteria.
However, when I try to find their Sequence type I'm finding that they aren't sequence typed.
I used SRST tool and in-house BLAST pipeline in order to check isolates' sequence type. Both these pipeline fail to identify isolates' sequence type information.
Used profile of E. coli#1 from pub mlst database.
I was wondering, what this could mean biologically about the data/genome?
I'm dealing with e. coli genome. I did de novo assembly using SPAdes 3.5, genome size is reasonable ~4.8+mbs.
Everything is sequenced, including plasmids of the bacteria.
However, when I try to find their Sequence type I'm finding that they aren't sequence typed.
I used SRST tool and in-house BLAST pipeline in order to check isolates' sequence type. Both these pipeline fail to identify isolates' sequence type information.
Used profile of E. coli#1 from pub mlst database.
I was wondering, what this could mean biologically about the data/genome?