Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • anna_vt
    Junior Member
    • Jun 2009
    • 5

    #1

    Understanding Maq log output

    Hi,

    I have a couple of questions about my Maq map output if anyone knows the answers I'd really appreciate them:

    Code:
    [match_search]  98% processed in 20717.101 sec: 31618863 / 21294334 = 1.485
    [match_search]  99% processed in 20765.187 sec: 28388678 / 20109661 = 1.412
    [match_search] 100% processed in 20768.725 sec: 1164880 / 1048335 = 1.111
    [match_core] sorting the hits and dumping the results...
    [ma_load_reads] loading reads...
    [ma_load_reads] 2000000*2 reads loaded.
    [mapping_count_single] 7, 11, 16, 27
    [maq_indel_pe] the indel detector only works with short-insert mate-pair reads.
    [match_data2mapping] 1922258 out of 4000000 raw reads are mapped with 0 in pairs.
    -- (total, isPE, mapped, paired) = (2000000, 0, 1922258, 0)
    First, what is the "mapping_count_single" - what do these 4 numbers mean?

    Second, this file contained 2,000,000 reads (8 million lines in fastq format), so how come the logfile says x out of 4000000 raw reads are mapped?

    Many Thanks
    Anna
  • Tally
    Member
    • Aug 2011
    • 12

    #2
    I have a similar issue. My output log reports n_reads*2 because they are paired end, but it looks like that is not the case for your data. My issue is the "x out of n raw reads are mapped" part:

    [match_data2mapping] 1976956 out of 2000000 raw reads are mapped with 1969400 in pairs.
    -- (total, isPE, mapped, paired) = (2000000, 1, 1976956, 1969400)

    What happened to the remaining 23,044 reads? It is not that they are simply unmapped, as I have a number of unmapped reads in my .sam alignment file.

    Any help much appreciated.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    31 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    22 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    39 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    45 views
    0 reactions
    Last Post SEQadmin2  
    Working...