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Dear all,
I'm using miRanalyzer to do the quantification analysis of miRNA. I have some questions confused me. I used the web server to analyze my data. It always shows that:An error occured with you job: 62512359 Please report it indicating the jobID. I checked my input file. I think there is no problem with it. It is unique sequence count file with tab separation.
AAAAAAAAAAAAAAAAAAAAAAA 2
AAAAAAAAAAAAAAAAAAAAAAAAAAATGAATTTTTGGGGGCCAAAGAAC 1
AAAAAAAAGACCGCCAGGGCT 1
AAAAAATTCCCTGAGGCCTCTG 1
AAAAACCGCAATGACTTTTG 4
AAAAACTGAGACTACTTTTG 3
AAAAACTGAGACTACTTTTGCA 2
AAAAACTGTAATTTCCTTTGCA 7
AAAAACTGTGATTACTTTTGC 1
I used two method to convert the fastq file to unique sequence count file. 1, I used the script groupReads.pl directly. 2, I used linux command awk'(NR%4==2){print}' reads.fastq|sort|uniq -c|awk '{print $2"\t'$1}'>read_for_miranalyzer.txt
Comparing these two methods, there are some different with the results.I think using linux command is better. But it also didn't work in the web server. Should I use stand-alone version?
Thank you in advance for your help!
I'm using miRanalyzer to do the quantification analysis of miRNA. I have some questions confused me. I used the web server to analyze my data. It always shows that:An error occured with you job: 62512359 Please report it indicating the jobID. I checked my input file. I think there is no problem with it. It is unique sequence count file with tab separation.
AAAAAAAAAAAAAAAAAAAAAAA 2
AAAAAAAAAAAAAAAAAAAAAAAAAAATGAATTTTTGGGGGCCAAAGAAC 1
AAAAAAAAGACCGCCAGGGCT 1
AAAAAATTCCCTGAGGCCTCTG 1
AAAAACCGCAATGACTTTTG 4
AAAAACTGAGACTACTTTTG 3
AAAAACTGAGACTACTTTTGCA 2
AAAAACTGTAATTTCCTTTGCA 7
AAAAACTGTGATTACTTTTGC 1
I used two method to convert the fastq file to unique sequence count file. 1, I used the script groupReads.pl directly. 2, I used linux command awk'(NR%4==2){print}' reads.fastq|sort|uniq -c|awk '{print $2"\t'$1}'>read_for_miranalyzer.txt
Comparing these two methods, there are some different with the results.I think using linux command is better. But it also didn't work in the web server. Should I use stand-alone version?
Thank you in advance for your help!