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  • lavik17
    Member
    • Nov 2013
    • 10
    #1

    Identifying a draft genome

    Hi everyone,
    I have a draft genome which was binned from a bacterial metagenome. I know for certain that the genome doesn't have a 16s gene in it, but should be fairly complete with 42 single copy bacterial genes present. I wish to identify the taxonomy of this genome. By BLASTing several ORFs in the genome against NCBI's nr database I have a reasonable idea to which taxon it belongs, but when running MAUVE to align my binned genome and a draft genome from NCBI I don't get a nicely re-ordered genome, only a mess...
    so my question has two parts:
    1. Is there an online tool to which I can upload my draft genome and it will run it again its database to produce similarity values to known genomes ?
    2. Which other tools would you recommend in order to asses the completeness of the genome ?

    Thanks!
    Tags: completeness, draft, genome, taxonomy
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