Hi,
I am doing Tophat/cufflinks assembly using RNA-seq data of six samples. I wonder which choice could be better, either pooled reads together of total six samples and then assembly once, or assembly six times and then using Cuffmerge for six assemblies?
Is there someone who had some experience?
Thank you!
Dawn
I am doing Tophat/cufflinks assembly using RNA-seq data of six samples. I wonder which choice could be better, either pooled reads together of total six samples and then assembly once, or assembly six times and then using Cuffmerge for six assemblies?
Is there someone who had some experience?
Thank you!
Dawn