Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Help: Gene Copy Number for individual organism (not CNV)

    Greetings!
    I am trying to determine the copy number of certain genes found in an individual organism (i.e. how many copies does an organism have of 'x' gene) by using read depth analysis of WGS paired end read data. I'm extremely interested in learning if this can be done, and if so, how. I'd be incredibly thankful if anyone could help in this matter.
    Last edited by IKnowNothing; 10-11-2015, 06:17 PM.

  • #2
    Hello, I am trying to do the same. I was told I could map to the genome and then use bedtools on the long run, but I am a little confused on how to do this. I would also be grateful if anyone could provide an answer for this.

    Comment


    • #3
      Here is a past thread on this topic that may be useful: http://seqanswers.com/forums/showthread.php?t=41062

      Comment


      • #4
        Thanks! Wouldn't this method only give estimated values? I was hoping to be able to use read depth analysis to find amount of gene copies... I know it's possible, but I haven't seen any examples over the web on how to do it.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Latest Developments in Precision Medicine
          by seqadmin



          Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

          Somatic Genomics
          “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
          05-24-2024, 01:16 PM
        • seqadmin
          Recent Advances in Sequencing Analysis Tools
          by seqadmin


          The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
          05-06-2024, 07:48 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, Yesterday, 06:55 AM
        0 responses
        12 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 05-30-2024, 03:16 PM
        0 responses
        24 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 05-29-2024, 01:32 PM
        0 responses
        28 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 05-24-2024, 07:15 AM
        0 responses
        215 views
        0 likes
        Last Post seqadmin  
        Working...
        X